Gene synthesis and vector construction
DNA delivery into chloroplasts
Selection and shoot regeneration
Plant grown to maturity insoil and harvest seeds
Large scale planting and harvest leaves
Lyse chloroplasts and purify protein
Protein Characterisation (Quality Control)
2-5 grams per kilogram of leaf material.
c.1MT of biomass/year for 10mX10m
Gene silencing, position and epigenetic effects are absent in the chloroplast genetic system.
Therefore, there are unchanging levels of expression
Plant-based proteins expression has GRAS status and does not harbour harmful endotoxins or viral and prion contaminants, resulting in a simplified downstream process
A highly scalable expression system for a continuous and sustainable production of recombinant proteins